Hotspot

trnF-GAA-ndhJ

LSC · 51354-52095 · 742 bp

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Pi 0.0121

MarkerSeek score 63.60

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 742
variable_sites: 13
pi: 0.0121
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	TCATCCGCGCTTAAAACGTA	55.0	45.0	58.0	58.0	893-918 bp	1.0	83.6
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	TCATCCGCGCTTAAAACGTA	50.0	45.0	58.1	58.0	857-882 bp	1.0	83.3
trnF-GAA-ndhJ_p3	GGGATAGCTCAGCTGGTAGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.0	58.1	859-884 bp	1.0	83.1
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	823-848 bp	1.0	82.8
trnF-GAA-ndhJ_p5	CTGGTAGAGCAGAGGACTGA	TCATCCGCGCTTAAAACGTA	55.0	45.0	57.9	58.0	881-906 bp	1.0	82.5