Hotspot

trnL-UAA-trnF-GAA

LSC · 50907-51280 · 374 bp

Back to Pouteria

Pi 0.00891

MarkerSeek score 51.90

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 374
variable_sites: 5
pi: 0.00891
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	AATCGTGAGGGTTCAAGTCC	TCTACCAGCTGAGCTATCCC	50.0	55.0	57.5	58.0	423-428 bp	1.0	78.1
trnL-UAA-trnF-GAA_p2	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	459-464 bp	1.0	77.5
trnL-UAA-trnF-GAA_p3	AATCGTGAGGGTTCAAGTCC	TCAGTCCTCTGCTCTACCAG	50.0	55.0	57.5	57.9	435-440 bp	1.0	76.9
trnL-UAA-trnF-GAA_p4	AATCGTGAGGGTTCAAGTCC	GAACTGGTGACACGAGGATT	50.0	50.0	57.5	57.5	456-461 bp	1.0	74.3
trnL-UAA-trnF-GAA_p5	TTCAAGTCCCTCTATCCCCA	TCTACCAGCTGAGCTATCCC	50.0	55.0	57.0	58.0	412-417 bp	1.0	74.2