Hotspot

trnC-GCA-petN

LSC · 28959-29730 · 772 bp

Back to Pouteria

Pi 0.0112

MarkerSeek score 65.00

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 772
variable_sites: 13
pi: 0.0112
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTAACTA	50.0	50.0	58.4	58.2	841-849 bp	1.0	77.6
trnC-GCA-petN_p2	AGTTCAAATCTGGGTGTCGC	AGCCCAAGCGAGACTAACTA	50.0	50.0	58.5	58.2	816-824 bp	1.0	76.7
trnC-GCA-petN_p3	GGGGGACTGCAAATCCTTTT	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.4	57.6	902-910 bp	1.0	75.9
trnC-GCA-petN_p4	TATCCTTTTGGCGACATGGC	AGCCCAAGCGAGACTAACTA	50.0	50.0	58.6	58.2	874-882 bp	1.0	75.5
trnC-GCA-petN_p5	AGTTCAAATCTGGGTGTCGC	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.5	57.6	877-885 bp	1.0	75.0