Hotspot

atpF-atpH

LSC · 13622-13992 · 371 bp

Back to Pouteria

Pi 0.00898

MarkerSeek score 64.00

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 371
variable_sites: 5
pi: 0.00898
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	ATTAAACCCGAAACTCCCGG	AGGCGGAAGGAAAAATACGA	50.0	45.0	57.9	57.2	550-561 bp	1.0	75.6
atpF-atpH_p2	ATTAAACCCGAAACTCCCGG	GGACTGGTTGTAGCATTAGCA	50.0	47.6	57.9	58.0	482-493 bp	1.0	74.1
atpF-atpH_p3	ATTAAACCCGAAACTCCCGG	TGGACTGGTTGTAGCATTAGC	50.0	47.6	57.9	58.0	483-494 bp	1.0	74.1
atpF-atpH_p4	ATTAAACCCGAAACTCCCGG	GCGGAAGGAAAAATACGAGG	50.0	50.0	57.9	56.9	548-559 bp	1.0	72.9
atpF-atpH_p5	ATTAAACCCGAAACTCCCGG	GGCGGAAGGAAAAATACGAG	50.0	50.0	57.9	56.9	549-560 bp	1.0	72.9