Hotspot

trnH-GUG-psbA

LSC · 95-614 · 520 bp

Back to Pouteria

Pi 0.0149

MarkerSeek score 58.10

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 520
variable_sites: 11
pi: 0.0149
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CTGCCTTAATCCACTTGGCT	GCTCCAGCTACAAATGGATA	50.0	45.0	57.9	54.9	569-599 bp	1.0	58.4
trnH-GUG-psbA_p2	ACTGCCTTAATCCACTTGGC	GCTCCAGCTACAAATGGATA	50.0	45.0	58.2	54.9	570-600 bp	1.0	58.2
trnH-GUG-psbA_p3	CTGCCTTAATCCACTTGGCT	GCTCCAGCTACAAATGGATAA	50.0	42.9	57.9	55.7	569-599 bp	1.0	57.0
trnH-GUG-psbA_p4	AATCCACTTGGCTACATCCG	GCTCCAGCTACAAATGGATA	50.0	45.0	57.7	54.9	562-592 bp	1.0	56.9
trnH-GUG-psbA_p5	ACTGCCTTAATCCACTTGGC	GCTCCAGCTACAAATGGATAA	50.0	42.9	58.2	55.7	570-600 bp	1.0	56.8