Hotspot

ndhG-ndhI

SSC · 124228-124640 · 413 bp

Back to Pourthiaea

Pi 0.00193

MarkerSeek score 51.20

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 413
variable_sites: 3
pi: 0.00193
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 51.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	AATCATGTATTGGTCCGGGC	TCAAATTGCTTTGGGTCGTT	50.0	40.0	58.0	56.4	80-529 bp	1.0	71.9
ndhG-ndhI_p2	AATACCACCCCCATACCTCC	TCAAATTGCTTTGGGTCGTT	55.0	40.0	58.2	56.4	80-584 bp	1.0	70.6
ndhG-ndhI_p3	TGTATTGGTCCGGGCAAATC	TCAAATTGCTTTGGGTCGTT	50.0	40.0	58.2	56.4	80-524 bp	1.0	70.1
ndhG-ndhI_p4	AATCATGTATTGGTCCGGGC	ATCAAATTGCTTTGGGTCGT	50.0	40.0	58.0	56.2	80-530 bp	1.0	70.1
ndhG-ndhI_p5	AATCATGTATTGGTCCGGGC	TCAAATTGCTTTGGGTCGTTT	50.0	38.1	58.0	57.2	80-529 bp	1.0	69.9