Hotspot

ndhF-rpl32

SSC · 116984-118037 · 1054 bp

Back to Pourthiaea

Pi 0.00462
MarkerSeek score 44.90
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1054
variable_sites
18
pi
0.00462
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
44.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TTGTTGCCGTTGGAAAAAGT TGAAATAGAGGTGCGCTTTT 40.0 40.0 57.0 55.7 80-1246 bp 1.0 55.4
ndhF-rpl32_p2 TTGTTGCCGTTGGAAAAAGT TTGAAATAGAGGTGCGCTTT 40.0 40.0 57.0 55.7 80-1247 bp 1.0 55.4
ndhF-rpl32_p3 TTGTTGCCGTTGGAAAAAGT TTTGAAATAGAGGTGCGCTT 40.0 40.0 57.0 55.7 80-1248 bp 1.0 55.4
ndhF-rpl32_p4 TTGTTGCCGTTGGAAAAAGT TTTTGAAATAGAGGTGCGCT 40.0 40.0 57.0 55.7 80-1249 bp 1.0 55.4
ndhF-rpl32_p5 TTGTTGCCGTTGGAAAAAGT TGAAATAGAGGTGCGCTTTTT 40.0 38.1 57.0 56.4 80-1246 bp 1.0 53.7