Hotspot

rpl33-rps18

LSC · 71694-71960 · 267 bp

Back to Pourthiaea

Pi 0.00748
MarkerSeek score 48.80
Primer available yes
Label rpl33-rps18

Feature Metadata

label_name
rpl33-rps18
feature_type
igs
region
LSC
length_bp
267
variable_sites
4
pi
0.00748
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.85
markerseek_score
48.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl33-rps18_p1 CGACACAATACGCCTAGTCG TCAATTCGATCCCCCGATTG 55.0 50.0 58.5 57.7 80-440 bp 1.0 78.5
rpl33-rps18_p2 GACACAATACGCCTAGTCGA TCAATTCGATCCCCCGATTG 50.0 50.0 57.2 57.7 80-439 bp 1.0 76.1
rpl33-rps18_p3 TCGACACAATACGCCTAGTC TCAATTCGATCCCCCGATTG 50.0 50.0 57.2 57.7 80-441 bp 1.0 76.1
rpl33-rps18_p4 CGACACAATACGCCTAGTCG ATTCGATCCCCCGATTGGAT 55.0 50.0 58.5 58.6 80-437 bp 1.0 75.5
rpl33-rps18_p5 AGAATCGACACAATACGCCT TCAATTCGATCCCCCGATTG 45.0 50.0 57.0 57.7 80-445 bp 1.0 75.1