Hotspot

trnT-GGU-psbD

LSC · 34318-35919 · 1602 bp

Back to Pourthiaea

Pi 0.0116
MarkerSeek score 64.10
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1602
variable_sites
58
pi
0.0116
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.96
markerseek_score
64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG GGCCAGACCAACCTACAAAA 55.0 50.0 58.1 58.0 80-1859 bp 1.0 86.4
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA GGCCAGACCAACCTACAAAA 50.0 50.0 58.1 58.0 80-1862 bp 1.0 86.2
trnT-GGU-psbD_p3 GTGGTAGAGTAACGCCATGG TAGGCCAGACCAACCTACAA 55.0 50.0 58.1 58.0 80-1861 bp 1.0 86.2
trnT-GGU-psbD_p4 GTGGTAGAGTAACGCCATGG ACCTACAAAAACGAAGCGGT 55.0 45.0 58.1 58.0 80-1849 bp 1.0 86.2
trnT-GGU-psbD_p5 TCAGTGGTAGAGTAACGCCA TAGGCCAGACCAACCTACAA 50.0 50.0 58.1 58.0 80-1864 bp 1.0 86.0