Hotspot

psbM-trnD-GUC

LSC · 31733-32977 · 1245 bp

Back to Pourthiaea

Pi 0.0055

MarkerSeek score 48.70

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1245
variable_sites: 17
pi: 0.0055
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 48.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	TCACCTTGGCTGACTGTTTT	TTCAATTGGTCAGAGCACCG	45.0	50.0	57.6	58.5	80-1410 bp	1.0	74.6
psbM-trnD-GUC_p2	AATCACCTTGGCTGACTGTT	TTCAATTGGTCAGAGCACCG	45.0	50.0	57.3	58.5	80-1412 bp	1.0	72.7
psbM-trnD-GUC_p3	ATCACCTTGGCTGACTGTTT	TTCAATTGGTCAGAGCACCG	45.0	50.0	57.3	58.5	80-1411 bp	1.0	72.7
psbM-trnD-GUC_p4	TCACCTTGGCTGACTGTTTT	GTTCAATTGGTCAGAGCACC	45.0	50.0	57.6	57.0	80-1411 bp	1.0	70.4
psbM-trnD-GUC_p5	ATCACCTTGGCTGACTGTTTT	TTCAATTGGTCAGAGCACCG	42.9	50.0	58.1	58.5	80-1411 bp	1.0	69.7