primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	GCGCTAACCTTGGTATGGAA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.161600	156	416	378-391	1.000000	81.8
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.165962	156	416	392-405	1.000000	81.7
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	ATATTATGGGCGAACGACGG	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.140446	156	416	389-402	0.875000	76.9
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ATATTATGGGCGAACGACGG	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.107227	156	416	439-452	0.875000	76.8
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	ATATTATGGGCGAACGACGG	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.149276	156	416	392-405	0.875000	76.8
trnK-UUU_p1	trnK-UUU	1	ACCCATGTCAACCAATACCG	TTGGTTTGGATTGCGTTGTG	20	20	50.0	45.0	57.9	57.8	0.0	0.0	0.338690	1727	4287	2760-2779	1.000000	77.7
trnK-UUU_p2	trnK-UUU	2	CCCATGTCAACCAATACCGA	TTGGTTTGGATTGCGTTGTG	20	20	50.0	45.0	57.6	57.8	0.0	0.0	0.627535	1727	4287	2759-2778	1.000000	75.4
trnK-UUU_p3	trnK-UUU	3	ACCCATGTCAACCAATACCG	TGATTGGTTTGGATTGCGTTG	20	21	50.0	42.9	57.9	58.0	0.0	0.0	1.146729	1727	4287	2763-2782	1.000000	71.2
trnK-UUU_p4	trnK-UUU	4	ACCCATGTCAACCAATACCG	GATTGGTTTGGATTGCGTTGT	20	21	50.0	42.9	57.9	58.0	0.0	0.0	1.148070	1727	4287	2762-2781	1.000000	71.2
trnK-UUU_p5	trnK-UUU	5	CCCATGTCAACCAATACCGAT	TTGGTTTGGATTGCGTTGTG	21	20	47.6	45.0	58.1	57.8	0.0	0.0	1.284957	1727	4287	2759-2778	1.000000	70.1
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AAAGCCGAGTACTCTACCGT	CGGAACTTCGCCCTAATCAA	20	20	50.0	50.0	58.2	58.0	20.3	0.0	0.186962	4288	5235	1020-1023	1.000000	79.0
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAGCCGAGTACTCTACCGTT	CGGAACTTCGCCCTAATCAA	20	20	50.0	50.0	58.2	58.0	20.3	0.0	0.186962	4288	5235	1019-1022	1.000000	79.0
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	CCGCACTTAAAAGCCGAGTA	CGGAACTTCGCCCTAATCAA	20	20	50.0	50.0	58.3	58.0	5.3	0.0	0.301348	4288	5235	1029-1032	1.000000	78.1
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AAAGCCGAGTACTCTACCGT	GGTGCTCAACCTACAGGAAC	20	20	50.0	55.0	58.2	58.2	20.3	1.3	0.358736	4288	5235	1075-1078	1.000000	77.6
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AAGCCGAGTACTCTACCGTT	GGTGCTCAACCTACAGGAAC	20	20	50.0	55.0	58.2	58.2	20.3	1.3	0.358736	4288	5235	1074-1077	1.000000	77.6
trnR-UCU-atpA_p1	trnR-UCU-atpA	1	GGAATGAAAAGCGTCCATTGT	AGACATTTACCGACGAAGCG	21	20	42.9	50.0	57.4	58.4	5.3	0.0	1.950350	10153	11058	495-1074	1.000000	72.0
trnR-UCU-atpA_p2	trnR-UCU-atpA	2	GGTTCAAATCCTATTGGACGC	AGACATTTACCGACGAAGCG	21	20	47.6	50.0	57.3	58.4	0.0	0.0	2.067175	10153	11058	435-1014	1.000000	71.3
trnR-UCU-atpA_p3	trnR-UCU-atpA	3	TGGATAGGACAGAGGTCTTCT	AGACATTTACCGACGAAGCG	21	20	47.6	50.0	56.9	58.4	0.0	0.0	2.425989	10153	11058	470-1049	1.000000	68.3
trnR-UCU-atpA_p4	trnR-UCU-atpA	4	GGAATGAAAAGCGTCCATTGT	GGAAGCCCTTTTGAAAGAAGC	21	21	42.9	47.6	57.4	58.0	5.3	5.1	2.607983	10153	11058	496-1055	1.000000	66.8
trnR-UCU-atpA_p5	trnR-UCU-atpA	5	GGAATGAAAAGCGTCCATTGT	ACCAAGACATTTACCGACGA	21	20	42.9	45.0	57.4	56.9	5.3	0.0	2.689422	10153	11058	495-1078	1.000000	66.0
atpH-atpI_p1	atpH-atpI	1	AATAGAAGCAAGCCCGACAG	GCGAATCCATGGAAGGTCAT	20	20	50.0	50.0	58.0	58.0	0.0	20.7	0.056966	14607	15785	80-1271	1.000000	81.0
atpH-atpI_p2	atpH-atpI	2	ATAACGGAAGCGGCAGAAAT	GCGAATCCATGGAAGGTCAT	20	20	45.0	50.0	58.0	58.0	1.4	20.7	0.065515	14607	15785	80-1237	1.000000	80.9
atpH-atpI_p3	atpH-atpI	3	AATAGAAGCAAGCCCGACAG	TTTTTGCAACCTTAGCTGCG	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.162578	14607	15785	80-1301	1.000000	80.1
atpH-atpI_p4	atpH-atpI	4	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACCTTAGCTGCG	20	20	45.0	45.0	58.0	57.9	1.4	0.0	0.171127	14607	15785	80-1267	1.000000	80.1
atpH-atpI_p5	atpH-atpI	5	AATAACGGAAGCGGCAGAAA	GCGAATCCATGGAAGGTCAT	20	20	45.0	50.0	58.2	58.0	1.4	20.7	0.211053	14607	15785	80-1238	1.000000	79.8
petN-psbM_p1	petN-psbM	1	AGTAAATCTCGCTTGGGCTG	AAAACAGTCAGCCAAGGTGA	20	20	50.0	45.0	58.0	57.6	0.0	0.0	0.465211	30388	31627	80-1438	1.000000	77.7
petN-psbM_p2	petN-psbM	2	AGTATGGGGAAGGAGTGGAC	AAAACAGTCAGCCAAGGTGA	20	20	55.0	45.0	58.1	57.6	0.0	0.0	0.554432	30388	31627	80-1381	1.000000	77.1
petN-psbM_p3	petN-psbM	3	GTATGGGGAAGGAGTGGACT	AAAACAGTCAGCCAAGGTGA	20	20	55.0	45.0	58.1	57.6	0.0	0.0	0.554432	30388	31627	80-1380	1.000000	77.1
petN-psbM_p4	petN-psbM	4	TATGGGGAAGGAGTGGACTC	AAAACAGTCAGCCAAGGTGA	20	20	55.0	45.0	57.8	57.6	0.0	0.0	0.611620	30388	31627	80-1379	1.000000	76.6
petN-psbM_p5	petN-psbM	5	GGGGAAGGAGTGGACTCTAG	AAAACAGTCAGCCAAGGTGA	20	20	60.0	45.0	58.2	57.6	6.7	0.0	0.652301	30388	31627	80-1376	1.000000	76.3
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	TCACCTTGGCTGACTGTTTT	TTCAATTGGTCAGAGCACCG	20	20	45.0	50.0	57.6	58.5	0.0	5.4	0.912704	31733	32977	80-1410	1.000000	74.6
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	AATCACCTTGGCTGACTGTT	TTCAATTGGTCAGAGCACCG	20	20	45.0	50.0	57.3	58.5	0.0	5.4	1.141734	31733	32977	80-1412	1.000000	72.7
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	ATCACCTTGGCTGACTGTTT	TTCAATTGGTCAGAGCACCG	20	20	45.0	50.0	57.3	58.5	0.0	5.4	1.141734	31733	32977	80-1411	1.000000	72.7
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	TCACCTTGGCTGACTGTTTT	GTTCAATTGGTCAGAGCACC	20	20	45.0	50.0	57.6	57.0	0.0	5.4	1.432858	31733	32977	80-1411	1.000000	70.4
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	ATCACCTTGGCTGACTGTTTT	TTCAATTGGTCAGAGCACCG	21	20	42.9	50.0	58.1	58.5	0.0	5.4	1.527884	31733	32977	80-1411	1.000000	69.7
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GTGGTAGAGTAACGCCATGG	GGCCAGACCAACCTACAAAA	20	20	55.0	50.0	58.1	58.0	7.6	0.0	0.074361	34318	35919	80-1859	1.000000	86.4
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	TCAGTGGTAGAGTAACGCCA	GGCCAGACCAACCTACAAAA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.096516	34318	35919	80-1862	1.000000	86.2
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	GTGGTAGAGTAACGCCATGG	TAGGCCAGACCAACCTACAA	20	20	55.0	50.0	58.1	58.0	7.6	5.0	0.097032	34318	35919	80-1861	1.000000	86.2
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	GTGGTAGAGTAACGCCATGG	ACCTACAAAAACGAAGCGGT	20	20	55.0	45.0	58.1	58.0	7.6	0.0	0.106399	34318	35919	80-1849	1.000000	86.2
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	TCAGTGGTAGAGTAACGCCA	TAGGCCAGACCAACCTACAA	20	20	50.0	50.0	58.1	58.0	0.0	5.0	0.119187	34318	35919	80-1864	1.000000	86.0
psaA-ycf3_p1	psaA-ycf3	1	GCCCATTCCTCGAAAGAAGT	TGGAAACAAGCAATAGCGCT	20	20	50.0	45.0	57.9	58.5	4.1	17.3	0.581718	44987	45721	80-896	1.000000	78.6
psaA-ycf3_p2	psaA-ycf3	2	GCCCATTCCTCGAAAGAAGT	CAATAGCGCTTACTCCTGGT	20	20	50.0	50.0	57.9	57.4	4.1	18.7	0.719389	44987	45721	80-886	1.000000	77.5
psaA-ycf3_p3	psaA-ycf3	3	GCCCATTCCTCGAAAGAAGT	GGAGGCTTGGTTCGATCAAG	20	20	50.0	55.0	57.9	58.6	4.1	18.9	0.741772	44987	45721	80-927	1.000000	77.2
psaA-ycf3_p4	psaA-ycf3	4	GCCCATTCCTCGAAAGAAGT	GCAATAGCGCTTACTCCTGG	20	20	50.0	55.0	57.9	58.8	4.1	18.7	0.893928	44987	45721	80-887	1.000000	76.1
psaA-ycf3_p5	psaA-ycf3	5	TTGAGAAATGACCCGGTCTG	TGGAAACAAGCAATAGCGCT	20	20	50.0	45.0	57.5	58.5	18.5	17.3	0.939070	44987	45721	80-916	1.000000	75.6
ycf3_p1	ycf3	1	ACACATACAAAGAGACCCGC	AAATTCATGAACCGTCCCCC	20	20	50.0	50.0	57.9	58.2	0.0	11.1	0.247434	45722	47712	80-2907	1.000000	79.9
ycf3_p2	ycf3	2	CATACAAAGAGACCCGCCAA	AAATTCATGAACCGTCCCCC	20	20	50.0	50.0	57.9	58.2	0.0	11.1	0.260622	45722	47712	80-2904	1.000000	79.8
ycf3_p3	ycf3	3	TCTGGGCTTTAATTGACGCA	ATGTTTGCGAAGATGGGGTT	20	20	45.0	45.0	57.8	58.1	1.2	0.0	0.269308	45722	47712	80-2906	1.000000	79.8
ycf3_p4	ycf3	4	GATCTGGAAGTTGATCCGGG	ATGTTTGCGAAGATGGGGTT	20	20	55.0	45.0	57.8	58.1	0.0	0.0	0.323363	45722	47712	80-2998	1.000000	79.3
ycf3_p5	ycf3	5	TCTGGGCTTTAATTGACGCA	GGGTTTCCGCCCTTTCTAAT	20	20	45.0	50.0	57.8	57.9	1.2	0.0	0.331987	45722	47712	80-2891	1.000000	79.3
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	49805	51017	80-1296	1.000000	81.0
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	49805	51017	80-1304	1.000000	80.4
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	49805	51017	80-1328	1.000000	80.1
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	57.8	58.1	15.5	0.0	0.309926	49805	51017	80-1336	1.000000	79.5
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GCGATGCTCTAACCTCTGAG	AGTCCGTAGCGTCTACCAAT	20	20	55.0	50.0	57.9	58.2	0.0	10.2	0.372548	49805	51017	80-1310	1.000000	79.1
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	GGGGCTAAAACTCCGGAAAT	GCACGTTGGGTCTTTGAAAC	20	20	50.0	50.0	57.9	58.2	5.2	0.0	0.304202	54505	55080	80-771	1.000000	84.4
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	GGGGCTAAAACTCCGGAAAT	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.9	57.8	5.2	0.0	0.336606	54505	55080	80-707	1.000000	84.4
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	GGGGCTAAAACTCCGGAAAT	AATGCACGTTGGGTCTTTGA	20	20	50.0	45.0	57.9	58.3	5.2	0.0	0.452969	54505	55080	80-774	1.000000	83.2
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	GGGGCTAAAACTCCGGAAAT	ATGCACGTTGGGTCTTTGAA	20	20	50.0	45.0	57.9	58.3	5.2	0.0	0.452969	54505	55080	80-773	1.000000	83.2
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	CTGGTCCTTTGCTAATCGGG	GCACGTTGGGTCTTTGAAAC	20	20	55.0	50.0	58.3	58.2	0.0	0.0	0.496460	54505	55080	80-788	1.000000	82.8
psbE-petL_p1	psbE-petL	1	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGCTCAGACCG	20	20	50.0	55.0	57.8	58.2	24.9	0.0	0.327511	68428	69685	80-1378	1.000000	78.0
psbE-petL_p2	psbE-petL	2	ACGAATAACCAACCCGCAAT	GTCGTATCTTGCTCAGACCG	20	20	45.0	55.0	58.2	58.2	0.0	0.0	0.342189	68428	69685	80-1464	1.000000	77.9
psbE-petL_p3	psbE-petL	3	TGCTGACGAATAACCAACCC	GTCGTATCTTGCTCAGACCG	20	20	50.0	55.0	58.2	58.2	0.0	0.0	0.355094	68428	69685	80-1469	1.000000	77.8
psbE-petL_p4	psbE-petL	4	GTTCTCCTGTGCTTCCAGAC	GTCGTATCTTGCTCAGACCG	20	20	55.0	55.0	58.2	58.2	0.9	0.0	0.369099	68428	69685	80-1373	1.000000	77.7
psbE-petL_p5	psbE-petL	5	AGAACGTTCTCCTGTGCTTC	TCGTATCTTGCTCAGACCGA	20	20	50.0	50.0	57.8	58.3	24.9	6.2	0.409873	68428	69685	80-1377	1.000000	77.4
rpl33-rps18_p1	rpl33-rps18	1	CGACACAATACGCCTAGTCG	TCAATTCGATCCCCCGATTG	20	20	55.0	50.0	58.5	57.7	0.0	0.0	0.790741	71694	71960	80-440	1.000000	78.5
rpl33-rps18_p2	rpl33-rps18	2	GACACAATACGCCTAGTCGA	TCAATTCGATCCCCCGATTG	20	20	50.0	50.0	57.2	57.7	0.0	0.0	1.092932	71694	71960	80-439	1.000000	76.1
rpl33-rps18_p3	rpl33-rps18	3	TCGACACAATACGCCTAGTC	TCAATTCGATCCCCCGATTG	20	20	50.0	50.0	57.2	57.7	0.0	0.0	1.092932	71694	71960	80-441	1.000000	76.1
rpl33-rps18_p4	rpl33-rps18	4	CGACACAATACGCCTAGTCG	ATTCGATCCCCCGATTGGAT	20	20	55.0	50.0	58.5	58.6	0.0	0.0	1.173970	71694	71960	80-437	1.000000	75.5
rpl33-rps18_p5	rpl33-rps18	5	AGAATCGACACAATACGCCT	TCAATTCGATCCCCCGATTG	20	20	45.0	50.0	57.0	57.7	0.0	0.0	1.216072	71694	71960	80-445	1.000000	75.1
ndhF-rpl32_p1	ndhF-rpl32	1	TTGTTGCCGTTGGAAAAAGT	TGAAATAGAGGTGCGCTTTT	20	20	40.0	40.0	57.0	55.7	0.0	9.7	3.383378	116984	118037	80-1246	1.000000	55.4
ndhF-rpl32_p2	ndhF-rpl32	2	TTGTTGCCGTTGGAAAAAGT	TTGAAATAGAGGTGCGCTTT	20	20	40.0	40.0	57.0	55.7	0.0	9.7	3.383378	116984	118037	80-1247	1.000000	55.4
ndhF-rpl32_p3	ndhF-rpl32	3	TTGTTGCCGTTGGAAAAAGT	TTTGAAATAGAGGTGCGCTT	20	20	40.0	40.0	57.0	55.7	0.0	9.7	3.383378	116984	118037	80-1248	1.000000	55.4
ndhF-rpl32_p4	ndhF-rpl32	4	TTGTTGCCGTTGGAAAAAGT	TTTTGAAATAGAGGTGCGCT	20	20	40.0	40.0	57.0	55.7	0.0	9.7	3.383378	116984	118037	80-1249	1.000000	55.4
ndhF-rpl32_p5	ndhF-rpl32	5	TTGTTGCCGTTGGAAAAAGT	TGAAATAGAGGTGCGCTTTTT	20	21	40.0	38.1	57.0	56.4	0.0	9.7	3.600224	116984	118037	80-1246	1.000000	53.7
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GGGCAGCATTGAAAGCTTTT	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	57.8	58.0	14.0	0.0	0.182194	118197	119636	80-1590	1.000000	81.1
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GGGCAGCATTGAAAGCTTTT	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	57.8	58.0	14.0	0.0	0.183564	118197	119636	80-1591	1.000000	81.1
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	TAGGGCAGCATTGAAAGCTT	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	57.8	58.0	0.0	0.0	0.228483	118197	119636	80-1592	1.000000	80.8
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	TAGGGCAGCATTGAAAGCTT	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	57.8	58.0	0.0	0.0	0.229854	118197	119636	80-1593	1.000000	80.8
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GGGCAGCATTGAAAGCTTTT	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	57.8	57.9	14.0	0.0	0.298641	118197	119636	80-1600	1.000000	80.2
ndhG-ndhI_p1	ndhG-ndhI	1	AATCATGTATTGGTCCGGGC	TCAAATTGCTTTGGGTCGTT	20	20	50.0	40.0	58.0	56.4	0.0	0.0	1.576114	124228	124640	80-529	1.000000	71.9
ndhG-ndhI_p2	ndhG-ndhI	2	AATACCACCCCCATACCTCC	TCAAATTGCTTTGGGTCGTT	20	20	55.0	40.0	58.2	56.4	0.0	0.0	1.728233	124228	124640	80-584	1.000000	70.6
ndhG-ndhI_p3	ndhG-ndhI	3	TGTATTGGTCCGGGCAAATC	TCAAATTGCTTTGGGTCGTT	20	20	50.0	40.0	58.2	56.4	13.2	0.0	1.799688	124228	124640	80-524	1.000000	70.1
ndhG-ndhI_p4	ndhG-ndhI	4	AATCATGTATTGGTCCGGGC	ATCAAATTGCTTTGGGTCGT	20	20	50.0	40.0	58.0	56.2	0.0	0.0	1.807085	124228	124640	80-530	1.000000	70.1
ndhG-ndhI_p5	ndhG-ndhI	5	AATCATGTATTGGTCCGGGC	TCAAATTGCTTTGGGTCGTTT	20	21	50.0	38.1	58.0	57.2	0.0	0.0	1.829054	124228	124640	80-529	1.000000	69.9