Hotspot

rps16

LSC · 5233-6443 · 1211 bp

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Pi 0.0138
MarkerSeek score 57.80
Primer available yes
Label rps16

Feature Metadata

label_name
rps16
feature_type
gene
region
LSC
length_bp
1211
variable_sites
15
pi
0.0138
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.90
markerseek_score
57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16_p1 TTGTGTTGAATTGGCACGAC CGTTCCGACCAATCTGTTGA 45.0 50.0 57.5 58.2 1648-1775 bp 1.0 79.5
rps16_p2 TTGTGTTGAATTGGCACGAC TCGTTCCGACCAATCTGTTG 45.0 50.0 57.5 58.2 1649-1776 bp 1.0 79.5
rps16_p3 GGATGGGGATAAACGGATCC CGTTCCGACCAATCTGTTGA 55.0 50.0 57.5 58.2 1775-1905 bp 1.0 79.5
rps16_p4 ACTTGTGTTGAATTGGCACG CGTTCCGACCAATCTGTTGA 45.0 50.0 57.5 58.2 1650-1777 bp 1.0 79.4
rps16_p5 ACTTGTGTTGAATTGGCACG TCGTTCCGACCAATCTGTTG 45.0 50.0 57.5 58.2 1651-1778 bp 1.0 79.4