Hotspot

ndhE-psaC

IRa · 143251-144278 · 1028 bp

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Pi 0.00598
MarkerSeek score 64.40
Primer available yes
Label ndhE-psaC

Feature Metadata

label_name
ndhE-psaC
feature_type
igs
region
IRa
length_bp
1028
variable_sites
6
pi
0.00598
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhE-psaC_p1 CGTAACAGAAAATCGACGCG ACATCCCGACCAAGGTATCA 50.0 50.0 58.0 58.1 1175-1181 bp 1.0 80.7
ndhE-psaC_p2 CGTAACAGAAAATCGACGCG GCCCGTACACATTGAGTACA 50.0 50.0 58.0 57.6 1132-1138 bp 1.0 78.8
ndhE-psaC_p3 CGTAACAGAAAATCGACGCG AGCTTTACATCCCGACCAAG 50.0 50.0 58.0 57.6 1181-1187 bp 1.0 78.5
ndhE-psaC_p4 CGTAACAGAAAATCGACGCG GCTTTACATCCCGACCAAGG 50.0 55.0 58.0 58.6 1180-1186 bp 1.0 76.7
ndhE-psaC_p5 CGTAACAGAAAATCGACGCG CGCCCGTACACATTGAGTAC 50.0 55.0 58.0 58.7 1133-1139 bp 1.0 76.0