Hotspot

rps15-ndhH

SSC · 131072-137710 · 6639 bp

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Pi 0.0222
MarkerSeek score 52.70
Primer available yes
Label rps15-ndhH

Feature Metadata

label_name
rps15-ndhH
feature_type
igs
region
SSC
length_bp
6639
variable_sites
145
pi
0.0222
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
52.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ndhH_p1 TGGCTCTTTGAGGTACCTGA TGGTGAGTGCCGAAAAGAAA 50.0 45.0 58.0 58.0 864-892 bp 1.0 85.3
rps15-ndhH_p2 TGGCTCTTTGAGGTACCTGA TCTTTGGTGAGTGCCGAAAA 50.0 45.0 58.0 58.0 896 bp 0.5 59.4
rps15-ndhH_p3 TGGCTCTTTGAGGTACCTGA TTCTTTGGTGAGTGCCGAAA 50.0 45.0 58.0 58.0 897 bp 0.5 59.4
rps15-ndhH_p4 TGGCTCTTTGAGGTACCTGA TTGGTGAGTGCCGAAAAGAA 50.0 45.0 58.0 58.0 893 bp 0.5 59.4
rps15-ndhH_p5 TGGCTCTTTGAGGTACCTGA TTTGGTGAGTGCCGAAAAGA 50.0 45.0 58.0 58.0 894 bp 0.5 59.4