Hotspot

ndhH-rps15

IRb · 130690-130792 · 103 bp

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Pi 0.0638
MarkerSeek score 71.40
Primer available yes
Label ndhH-rps15

Feature Metadata

label_name
ndhH-rps15
feature_type
igs
region
IRb
length_bp
103
variable_sites
6
pi
0.0638
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.91
markerseek_score
71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhH-rps15_p1 TCCGGAAGCTCGTAACATTG TGGTGAGTGCCGAAAAGAAA 50.0 45.0 58.0 58.0 1253-1281 bp 1.0 83.6
ndhH-rps15_p2 TCCGGAAGCTCGTAACATTG TCTTTGGTGAGTGCCGAAAA 50.0 45.0 58.0 58.0 1285 bp 0.5 59.6
ndhH-rps15_p3 TCCGGAAGCTCGTAACATTG TTCTTTGGTGAGTGCCGAAA 50.0 45.0 58.0 58.0 1286 bp 0.5 59.6
ndhH-rps15_p4 TCCGGAAGCTCGTAACATTG TTGGTGAGTGCCGAAAAGAA 50.0 45.0 58.0 58.0 1282 bp 0.5 59.6
ndhH-rps15_p5 TCCGGAAGCTCGTAACATTG TTTGGTGAGTGCCGAAAAGA 50.0 45.0 58.0 58.0 1283 bp 0.5 59.6