Hotspot

psaC-ndhE

IRb · 124122-125149 · 1028 bp

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Pi 0.00598
MarkerSeek score 64.40
Primer available yes
Label psaC-ndhE

Feature Metadata

label_name
psaC-ndhE
feature_type
igs
region
IRb
length_bp
1028
variable_sites
6
pi
0.00598
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaC-ndhE_p1 ACATCCCGACCAAGGTATCA CGTAACAGAAAATCGACGCG 50.0 50.0 58.1 58.0 1175-1181 bp 1.0 80.7
psaC-ndhE_p2 GCCCGTACACATTGAGTACA CGTAACAGAAAATCGACGCG 50.0 50.0 57.6 58.0 1132-1138 bp 1.0 78.8
psaC-ndhE_p3 AGCTTTACATCCCGACCAAG CGTAACAGAAAATCGACGCG 50.0 50.0 57.6 58.0 1181-1187 bp 1.0 78.5
psaC-ndhE_p4 GCTTTACATCCCGACCAAGG CGTAACAGAAAATCGACGCG 55.0 50.0 58.6 58.0 1180-1186 bp 1.0 76.7
psaC-ndhE_p5 CGCCCGTACACATTGAGTAC CGTAACAGAAAATCGACGCG 55.0 50.0 58.7 58.0 1133-1139 bp 1.0 76.0