Hotspot

trnL-CAA-ndhB

LSC · 100431-101185 · 755 bp

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Pi 0.0328
MarkerSeek score 68.70
Primer available yes
Label trnL-CAA-ndhB

Feature Metadata

label_name
trnL-CAA-ndhB
feature_type
igs
region
LSC
length_bp
755
variable_sites
24
pi
0.0328
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-CAA-ndhB_p1 GTCTCGCGTGTCTACCATTT TGCAATTGCTCAGGATACCC 50.0 50.0 58.0 57.9 815 bp 0.5 59.5
trnL-CAA-ndhB_p2 CTCGCGTGTCTACCATTTCA TGCAATTGCTCAGGATACCC 50.0 50.0 58.0 57.9 813 bp 0.5 59.5
trnL-CAA-ndhB_p3 GCGTGTCTACCATTTCACCA TGCAATTGCTCAGGATACCC 50.0 50.0 58.2 57.9 810 bp 0.5 58.0
trnL-CAA-ndhB_p4 TCTCGCGTGTCTACCATTTC TGCAATTGCTCAGGATACCC 50.0 50.0 57.7 57.9 814 bp 0.5 57.4
trnL-CAA-ndhB_p5 TCGCGTGTCTACCATTTCAC TGCAATTGCTCAGGATACCC 50.0 50.0 58.3 57.9 812 bp 0.5 57.2