Hotspot

psbE-petL

LSC · 70568-71738 · 1171 bp

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Pi 0.00864
MarkerSeek score 64.80
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1171
variable_sites
10
pi
0.00864
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 TGACAAATAACCAACCCGCA AAGCAGCCAGTAGAAAACCA 45.0 45.0 58.0 57.3 1318-1329 bp 1.0 76.0
psbE-petL_p2 TGACAAATAACCAACCCGCA AGCAGCCAGTAGAAAACCAA 45.0 45.0 58.0 57.3 1317-1328 bp 1.0 76.0
psbE-petL_p3 TGACAAATAACCAACCCGCA AACCAATCAAAAGCGCTGAG 45.0 45.0 58.0 57.3 1348-1359 bp 1.0 75.5
psbE-petL_p4 TGACAAATAACCAACCCGCA CAATCAAAAGCGCTGAGGTT 45.0 45.0 58.0 57.3 1345-1356 bp 1.0 75.5
psbE-petL_p5 TGACAAATAACCAACCCGCA AAACCAATCAAAAGCGCTGAG 45.0 42.9 58.0 58.0 1349-1360 bp 1.0 73.1