Hotspot

accD-psaI

LSC · 63700-64768 · 1069 bp

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Pi 0.0216
MarkerSeek score 62.50
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
1069
variable_sites
23
pi
0.0216
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 TCAGCTCCATGGTTTCCTTC CCATTGCAATTGCCGGAAAT 50.0 45.0 57.5 58.0 1185-1250 bp 1.0 77.5
accD-psaI_p2 CAGCTCCATGGTTTCCTTCC CCATTGCAATTGCCGGAAAT 55.0 45.0 58.5 58.0 1184-1249 bp 1.0 77.2
accD-psaI_p3 TCAGCTCCATGGTTTCCTTC CGTCGCAGCTAGACAATCTT 50.0 50.0 57.5 58.1 1234-1299 bp 1.0 77.0
accD-psaI_p4 TCAGCTCCATGGTTTCCTTC AAGAAGCCATTGCAATTGCC 50.0 45.0 57.5 57.9 1191-1256 bp 1.0 76.9
accD-psaI_p5 CAGCTCCATGGTTTCCTTCC CGTCGCAGCTAGACAATCTT 55.0 50.0 58.5 58.1 1233-1298 bp 1.0 76.7