Hotspot

rps4-trnT-UGU

LSC · 49227-50191 · 965 bp

Back to Pothos

Pi 0.0234
MarkerSeek score 58.80
Primer available yes
Label rps4-trnT-UGU

Feature Metadata

label_name
rps4-trnT-UGU
feature_type
igs
region
LSC
length_bp
965
variable_sites
19
pi
0.0234
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.84
markerseek_score
58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps4-trnT-UGU_p1 AATTGTCGCTCTGTCAGACC CCGAACGAAACCAGAGGAAT 50.0 50.0 57.9 57.9 1780-1890 bp 1.0 83.7
rps4-trnT-UGU_p2 TGACCTGTTGATCCTTTGGC CCGAACGAAACCAGAGGAAT 50.0 50.0 58.1 57.9 1828-1938 bp 1.0 83.7
rps4-trnT-UGU_p3 TACCAGAGCGCAATTGGTTT CCGAACGAAACCAGAGGAAT 45.0 50.0 58.1 57.9 1704-1814 bp 1.0 83.7
rps4-trnT-UGU_p4 TTACCAGAGCGCAATTGGTT CCGAACGAAACCAGAGGAAT 45.0 50.0 58.1 57.9 1705-1815 bp 1.0 83.7
rps4-trnT-UGU_p5 TTTACCAGAGCGCAATTGGT CCGAACGAAACCAGAGGAAT 45.0 50.0 58.1 57.9 1706-1816 bp 1.0 83.7