Hotspot

psbZ-trnG-GCC

LSC · 38667-39124 · 458 bp

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Pi 0.0334
MarkerSeek score 71.30
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
458
variable_sites
13
pi
0.0334
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.85
markerseek_score
71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 TGCTTCTTCTGATGGTTGGT CTCTTCTTCCTAACCGTGGG 45.0 55.0 57.0 57.3 587-656 bp 1.0 72.6
psbZ-trnG-GCC_p2 GCTTCTTCTGATGGTTGGTCA CTCTTCTTCCTAACCGTGGG 47.6 55.0 58.2 57.3 586-655 bp 1.0 70.5
psbZ-trnG-GCC_p3 TGCTTCTTCTGATGGTTGGTC CTCTTCTTCCTAACCGTGGG 47.6 55.0 58.2 57.3 587-656 bp 1.0 70.5
psbZ-trnG-GCC_p4 TTGCTTCTTCTGATGGTTGGT CTCTTCTTCCTAACCGTGGG 42.9 55.0 57.8 57.3 588-657 bp 1.0 70.4
psbZ-trnG-GCC_p5 TGCTTCTTCTGATGGTTGGT CACTCTTCTTCCTAACCGTGG 45.0 52.4 57.0 58.0 589-658 bp 1.0 69.7