Hotspot

trnQ-UUG-psbK

LSC · 7818-8206 · 389 bp

Back to Pothos

Pi 0.0286
MarkerSeek score 64.30
Primer available yes
Label trnQ-UUG-psbK

Feature Metadata

label_name
trnQ-UUG-psbK
feature_type
igs
region
LSC
length_bp
389
variable_sites
11
pi
0.0286
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnQ-UUG-psbK_p1 GACCAAAACCCGTTGCCTTA CTCAGGCAATTTCCCGAAGA 50.0 50.0 58.7 57.9 514-516 bp 1.0 76.0
trnQ-UUG-psbK_p2 CGAATAACGGGACCAAAACC CTCAGGCAATTTCCCGAAGA 50.0 50.0 57.1 57.9 524-526 bp 1.0 74.1
trnQ-UUG-psbK_p3 ACCAAAACCCGTTGCCTTAC CTCAGGCAATTTCCCGAAGA 50.0 50.0 59.0 57.9 513-515 bp 1.0 73.7
trnQ-UUG-psbK_p4 GACCAAAACCCGTTGCCTTA TCAGGCAATTTCCCGAAGAA 50.0 45.0 58.7 57.4 513-515 bp 1.0 72.3
trnQ-UUG-psbK_p5 TGAAGAATTGATCTGGGACGG CTCAGGCAATTTCCCGAAGA 47.6 50.0 57.7 57.9 560-562 bp 1.0 71.1