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rps16

LSC · 5207-6330 · 1124 bp

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Pi 0.0177

MarkerSeek score 72.10

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1124
variable_sites: 209
pi: 0.0177
unique_haplotype_count: 90
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	ACTTGTGTTGGATTGGCACT	TGTACCAACCGAACCAATGA	45.0	45.0	57.9	57.1	1355-1553 bp	0.968421	82.7
rps16_p2	GAAGACGGGGGAGAAGTAGA	TGTACCAACCGAACCAATGA	55.0	45.0	57.9	57.1	1410-1478 bp	0.905263	79.5
rps16_p3	ACGAAAATCATCCCATCGGG	TGTACCAACCGAACCAATGA	50.0	45.0	58.0	57.1	1314-1369 bp	0.821053	76.7
rps16_p4	TAAGGAAGACGGGGGAGAAG	TGTACCAACCGAACCAATGA	55.0	45.0	58.1	57.1	1414-1482 bp	0.842105	76.6
rps16_p5	CCTCCTGGCTCTCTTCTCTT	TGTACCAACCGAACCAATGA	55.0	45.0	58.1	57.1	1825-1884 bp	0.536842	62.7