Hotspot

trnR-UCU-atpA

LSC · 10099-10738 · 640 bp

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Pi 0.0467
MarkerSeek score 82.30
Primer available yes
Label trnR-UCU-atpA

Feature Metadata

label_name
trnR-UCU-atpA
feature_type
igs
region
LSC
length_bp
640
variable_sites
257
pi
0.0467
unique_haplotype_count
84
primer_available
yes
alignment_reliability
0.96
markerseek_score
82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-atpA_p1 ATTGGAGTGAAAAGCGTCCA AGGAGCAAGTGGAACGTTTT 45.0 45.0 57.7 57.9 673-954 bp 0.947368 87.3
trnR-UCU-atpA_p2 TGGAGTGAAAAGCGTCCATT AGGAGCAAGTGGAACGTTTT 45.0 45.0 57.7 57.9 671-952 bp 0.947368 87.3
trnR-UCU-atpA_p3 TTGGAGTGAAAAGCGTCCAT AGGAGCAAGTGGAACGTTTT 45.0 45.0 57.7 57.9 672-953 bp 0.947368 87.3
trnR-UCU-atpA_p4 TGGAGTGAAAAGCGTCCATT ATTCAGGAGCAAGTGGAACG 45.0 50.0 57.7 58.2 675-956 bp 0.947368 86.2
trnR-UCU-atpA_p5 TTGGAGTGAAAAGCGTCCAT ATTCAGGAGCAAGTGGAACG 45.0 50.0 57.7 58.2 676-957 bp 0.947368 86.2