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trnK-UUU-rps16

LSC · 4363-5206 · 844 bp

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Pi 0.0283

MarkerSeek score 68.00

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 844
variable_sites: 227
pi: 0.0283
unique_haplotype_count: 70
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	TCAGGATCAGTCGTGGTCTT	CGACGAGAGGGAAGAGATCT	50.0	55.0	58.1	58.0	1059-1186 bp	1.0	91.1
trnK-UUU-rps16_p2	TTCAGGATCAGTCGTGGTCT	CGACGAGAGGGAAGAGATCT	50.0	55.0	58.1	58.0	1060-1187 bp	1.0	91.1
trnK-UUU-rps16_p3	AAAGCCGAGTACTCTACCGT	CGACGAGAGGGAAGAGATCT	50.0	55.0	58.2	58.0	968-1095 bp	1.0	90.3
trnK-UUU-rps16_p4	AAGCCGAGTACTCTACCGTT	CGACGAGAGGGAAGAGATCT	50.0	55.0	58.2	58.0	967-1094 bp	1.0	90.3
trnK-UUU-rps16_p5	GGACTCTCTAGAACCTCCCC	CGACGAGAGGGAAGAGATCT	60.0	55.0	57.9	58.0	1599-1697 bp	0.926316	88.1