Hotspot

ycf1

SSC · 126071-131461 · 5391 bp

Back to Potamogeton

Pi 0.00905

MarkerSeek score 47.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5391
variable_sites: 109
pi: 0.00905
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CCTCATACCACTCGGGAGAT	TCCGGCATTATACGCAACAA	55.0	45.0	58.0	58.0	441-447 bp	1.0	82.5
ycf1_p2	ATCCTCATACCACTCGGGAG	TTTAAGTGGACCATACCGCG	55.0	50.0	58.0	58.0	919-931 bp	1.0	82.4
ycf1_p3	CCTCATACCACTCGGGAGAT	TTTAAGTGGACCATACCGCG	55.0	50.0	58.0	58.0	917-929 bp	1.0	82.4
ycf1_p4	ATCCTCATACCACTCGGGAG	ACACGATTCATGGTCTGCTC	55.0	50.0	58.0	58.0	818 bp	0.2	47.9
ycf1_p5	CCTCATACCACTCGGGAGAT	ACACGATTCATGGTCTGCTC	55.0	50.0	58.0	58.0	816 bp	0.2	47.9