Hotspot

rps2

LSC · 16195-16911 · 717 bp

Back to Potamogeton

Pi 0.00335

MarkerSeek score 46.50

Primer available yes

Label rps2

Feature Metadata

label_name: rps2
feature_type: gene
region: LSC
length_bp: 717
variable_sites: 6
pi: 0.00335
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2_p1	GATAAGTACTTGGGCGTGCA	CTTGGTAGAAGGTTCCGTCG	50.0	55.0	58.0	58.0	1170-1194 bp	1.0	82.9
rps2_p2	TGATAAGTACTTGGGCGTGC	CTTGGTAGAAGGTTCCGTCG	50.0	55.0	58.0	58.0	1171-1195 bp	1.0	82.9
rps2_p3	GATAAGTACTTGGGCGTGCA	TCCTTGGTAGAAGGTTCCGT	50.0	50.0	58.0	58.0	1172-1196 bp	1.0	82.8
rps2_p4	TGATAAGTACTTGGGCGTGC	TCCTTGGTAGAAGGTTCCGT	50.0	50.0	58.0	58.0	1173-1197 bp	1.0	82.8
rps2_p5	GATTCCGAACAGCTATGGCA	CTTGGTAGAAGGTTCCGTCG	50.0	55.0	58.0	58.0	1228-1252 bp	1.0	82.5