Hotspot

rpl32-trnL-UAG

SSC · 115324-115936 · 613 bp

Back to Potamogeton

Pi 0.0178

MarkerSeek score 58.20

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 613
variable_sites: 22
pi: 0.0178
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	ATTTGTGGGCGGTTAAAGCT	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.4	58.0	731-778 bp	1.0	80.6
rpl32-trnL-UAG_p2	ATTTGTGGGCGGTTAAAGCT	CAGCGTGTCTACCGATTTCA	45.0	50.0	58.4	58.0	732-779 bp	1.0	80.6
rpl32-trnL-UAG_p3	ATTTGTGGGCGGTTAAAGCT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.4	57.9	741-788 bp	1.0	79.6
rpl32-trnL-UAG_p4	TGTTTTTGAATGGCGGTTCC	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.5	58.0	813-860 bp	1.0	79.2
rpl32-trnL-UAG_p5	ATTTGTGGGCGGTTAAAGCT	TTGGGATTGTAGAGCGGGTA	45.0	50.0	58.4	58.1	1266-1313 bp	1.0	78.5