Hotspot

ycf4-cemA

LSC · 62438-63047 · 610 bp

Back to Potamogeton

Pi 0.0152
MarkerSeek score 55.10
Primer available yes
Label ycf4-cemA

Feature Metadata

label_name
ycf4-cemA
feature_type
igs
region
LSC
length_bp
610
variable_sites
18
pi
0.0152
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf4-cemA_p1 TATGGGATGGCGGGTCTATT TAGAGAAATCCACCAGGGCA 50.0 50.0 58.0 58.0 1293-1303 bp 1.0 81.5
ycf4-cemA_p2 TATGGGATGGCGGGTCTATT CCCGAGATTCCACCAATGAG 50.0 55.0 58.0 58.0 1344-1354 bp 1.0 81.4
ycf4-cemA_p3 CGTTGGGGATTTCCTGGAAA TAGAGAAATCCACCAGGGCA 50.0 50.0 58.1 58.0 1182-1192 bp 1.0 81.4
ycf4-cemA_p4 TTTCGTTGGGGATTTCCTGG TAGAGAAATCCACCAGGGCA 50.0 50.0 58.1 58.0 1185-1195 bp 1.0 81.4
ycf4-cemA_p5 TATGGGATGGCGGGTCTATT GAGAAATCCACCAGGGCAAA 50.0 50.0 58.0 58.1 1291-1301 bp 1.0 81.2