Hotspot

accD-psaI

LSC · 60523-61387 · 865 bp

Back to Potamogeton

Pi 0.0101

MarkerSeek score 62.30

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 865
variable_sites: 19
pi: 0.0101
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TCAGCTCCATGGTTTCTTTTCT	CCGGAAAAACAAGGCCTACT	40.9	50.0	57.9	58.1	969-1006 bp	1.0	65.3
accD-psaI_p2	TCAGCTCCATGGTTTCTTTTCT	AAGAAGCCATTGCAATTGCC	40.9	45.0	57.9	57.9	987-1024 bp	1.0	65.2
accD-psaI_p3	TCAGCTCCATGGTTTCTTTTCT	CCATTGCAATTGCCGGAAAA	40.9	45.0	57.9	58.2	981-1018 bp	1.0	64.5
accD-psaI_p4	TCAGCTCCATGGTTTCTTTTCT	TGCAATTGCCGGAAAAACAA	40.9	40.0	57.9	57.7	977-1014 bp	1.0	63.5
accD-psaI_p5	TCAGCTCCATGGTTTCTTTTCT	TTGCAATTGCCGGAAAAACA	40.9	40.0	57.9	57.7	978-1015 bp	1.0	63.5