Hotspot

psbM-trnD-GUC

LSC · 30796-31829 · 1034 bp

Back to Potamogeton

Pi 0.0114
MarkerSeek score 57.40
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
1034
variable_sites
24
pi
0.0114
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.98
markerseek_score
57.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 AAAAAGCGGTAGGAACTAGA GTTCGAGCCCCGTCAGTC 40.0 66.7 54.0 60.1 1094-1120 bp 1.0 44.6
psbM-trnD-GUC_p2 AAAAGCGGTAGGAACTAGAA GTTCGAGCCCCGTCAGTC 40.0 66.7 54.0 60.1 1093-1119 bp 1.0 44.6
psbM-trnD-GUC_p3 AAAAAGCGGTAGGAACTAGAA GTTCGAGCCCCGTCAGTC 38.1 66.7 54.9 60.1 1094-1120 bp 1.0 44.6
psbM-trnD-GUC_p4 GCGGTAGGAACTAGAATAAAGAGT GTTCGAGCCCCGTCAGTC 41.7 66.7 57.9 60.1 1089-1115 bp 1.0 44.6
psbM-trnD-GUC_p5 AAAGCGGTAGGAACTAGAAT GTTCGAGCCCCGTCAGTC 40.0 66.7 53.8 60.1 1092-1118 bp 1.0 44.6