Hotspot

trnC-GCA-petN

LSC · 28810-29561 · 752 bp

Back to Potamogeton

Pi 0.0134

MarkerSeek score 63.00

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 752
variable_sites: 21
pi: 0.0134
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	AGTTCAAATCTGGGTGTCGC	ACTCCTTCCCCACACTACAA	50.0	50.0	58.5	57.9	844-899 bp	1.0	79.2
trnC-GCA-petN_p2	GTATCGTTTTGGCGACATGG	ACTCCTTCCCCACACTACAA	50.0	50.0	57.5	57.9	903-958 bp	1.0	79.1
trnC-GCA-petN_p3	AGTTCAAATCTGGGTGTCGC	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.5	58.2	790-845 bp	1.0	79.1
trnC-GCA-petN_p4	GTATCGTTTTGGCGACATGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	57.5	58.2	849-904 bp	1.0	79.0
trnC-GCA-petN_p5	GTATCGTTTTGGCGACATGG	CACACTACAAGCGAAAGGGA	50.0	50.0	57.5	57.8	893-948 bp	1.0	78.7