Hotspot

atpI-rps2

LSC · 15949-16194 · 246 bp

Back to Potamogeton

Pi 0.0337

MarkerSeek score 72.00

Primer available yes

Label atpI-rps2

Feature Metadata

label_name: atpI-rps2
feature_type: igs
region: LSC
length_bp: 246
variable_sites: 13
pi: 0.0337
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 72.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-rps2_p1	GGCCTACCTCTACACCTGAT	CGGCCAATGATGACGCTATA	55.0	50.0	57.9	57.9	401-425 bp	1.0	85.2
atpI-rps2_p2	GTTGGCCTACCTCTACACCT	CGGCCAATGATGACGCTATA	55.0	50.0	58.4	57.9	404-428 bp	1.0	82.4
atpI-rps2_p3	TTGGCCTACCTCTACACCTG	CGGCCAATGATGACGCTATA	55.0	50.0	58.4	57.9	403-427 bp	1.0	82.4
atpI-rps2_p4	GTGCACTTGTAAACCCCCTA	CGGCCAATGATGACGCTATA	50.0	50.0	57.4	57.9	439-463 bp	1.0	81.1
atpI-rps2_p5	GGCCTACCTCTACACCTGAT	GTATCCGCAGTTTGTGAGGG	55.0	55.0	57.9	58.6	351-375 bp	1.0	81.1