Hotspot

trnS-GCU-trnG-UCC

LSC · 8800-9662 · 863 bp

Back to Potamogeton

Pi 0.0113

MarkerSeek score 61.80

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 863
variable_sites: 19
pi: 0.0113
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	906-948 bp	1.0	56.5
trnS-GCU-trnG-UCC_p2	AATCCGACGCTTTAGTCCAC	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	905-947 bp	1.0	56.5
trnS-GCU-trnG-UCC_p3	CAATCCGACGCTTTAGTCCA	AGAACGAATCACACTTTTACCAC	50.0	39.1	58.0	57.3	909-951 bp	1.0	55.4
trnS-GCU-trnG-UCC_p4	CAATCCGACGCTTTAGTCCA	GAACGAATCACACTTTTACCACT	50.0	39.1	58.0	57.3	908-950 bp	1.0	55.4
trnS-GCU-trnG-UCC_p5	CGAACCCTCGGTACAAACAA	ACGAATCACACTTTTACCACT	50.0	38.1	58.1	55.5	943-985 bp	1.0	55.3