Hotspot

trnQ-UUG-psbK

LSC · 7107-7934 · 828 bp

Back to Potamogeton

Pi 0.0142

MarkerSeek score 60.40

Primer available yes

Label trnQ-UUG-psbK

Feature Metadata

label_name: trnQ-UUG-psbK
feature_type: igs
region: LSC
length_bp: 828
variable_sites: 23
pi: 0.0142
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-psbK_p1	CCTTACCACTTGGCCACG	CTCGAATGAGGGGCAGAATT	61.1	50.0	58.0	57.7	834-926 bp	1.0	67.2
trnQ-UUG-psbK_p2	GTTGCCTTACCACTTGGCC	CTCGAATGAGGGGCAGAATT	57.9	50.0	59.0	57.7	838-930 bp	1.0	67.1
trnQ-UUG-psbK_p3	GCCTTACCACTTGGCCAC	CTCGAATGAGGGGCAGAATT	61.1	50.0	58.3	57.7	835-927 bp	1.0	64.8
trnQ-UUG-psbK_p4	TTGCCTTACCACTTGGCCA	CTCGAATGAGGGGCAGAATT	52.6	50.0	59.5	57.7	837-929 bp	1.0	63.7
trnQ-UUG-psbK_p5	TTGCCTTACCACTTGGCC	CTCGAATGAGGGGCAGAATT	55.6	50.0	57.5	57.7	837-929 bp	1.0	63.2