Hotspot

trnI-CAU-trnH-GUG

IRa · 155582-156125 · 544 bp

Back to Portulaca

Pi 0.151
MarkerSeek score 76.00
Primer available yes
Label trnI-CAU-trnH-GUG

Feature Metadata

label_name
trnI-CAU-trnH-GUG
feature_type
igs
region
IRa
length_bp
544
variable_sites
86
pi
0.151
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.81
markerseek_score
76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnI-CAU-trnH-GUG_p1 CAACGGGACCCTCCAATAAG AGAAGAGTTCGACCCAATGC 55.0 50.0 58.0 57.9 289-471 bp 1.0 90.9
trnI-CAU-trnH-GUG_p2 CAACGGGACCCTCCAATAAG GAAGAGTTCGACCCAATGCT 55.0 50.0 58.0 57.9 288-470 bp 1.0 90.9
trnI-CAU-trnH-GUG_p3 AACGGGACCCTCCAATAAGT AGAAGAGTTCGACCCAATGC 50.0 50.0 58.3 57.9 288-470 bp 1.0 88.5
trnI-CAU-trnH-GUG_p4 AACGGGACCCTCCAATAAGT GAAGAGTTCGACCCAATGCT 50.0 50.0 58.3 57.9 287-469 bp 1.0 88.5
trnI-CAU-trnH-GUG_p5 CAACGGGACCCTCCAATAAG CCAAAGAAGAGTTCGACCCA 55.0 50.0 58.0 57.5 293-475 bp 1.0 87.3