Hotspot

trnN-GUU-ycf1

IRb · 108628-108973 · 346 bp

Back to Portulaca

Pi 0.0838

MarkerSeek score 79.20

Primer available yes

Label trnN-GUU-ycf1

Feature Metadata

label_name: trnN-GUU-ycf1
feature_type: igs
region: IRb
length_bp: 346
variable_sites: 37
pi: 0.0838
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ycf1_p1	GGCAGAATAGCAGAGCAAGT	CCTGTGGAGAATGTGGTCAG	50.0	55.0	58.0	57.9	873-1353 bp	1.0	86.2
trnN-GUU-ycf1_p2	GAACTTGGGTGTGGGTCTTT	CCTGTGGAGAATGTGGTCAG	50.0	55.0	57.9	57.9	1025-1515 bp	1.0	85.9
trnN-GUU-ycf1_p3	GGCAGAATAGCAGAGCAAGT	AGGAAGAGATAAGAGGGCCC	50.0	55.0	58.0	57.9	891-926 bp	0.666667	70.4
trnN-GUU-ycf1_p4	GGCAGAATAGCAGAGCAAGT	GGAAGAGATAAGAGGGCCCT	50.0	55.0	58.0	57.9	890-925 bp	0.666667	70.4
trnN-GUU-ycf1_p5	GGCAGAATAGCAGAGCAAGT	TTCCATAACCAGAGCTCGGA	50.0	50.0	58.0	58.1	913-948 bp	0.666667	70.2