Hotspot

clpP-psbB

LSC · 73286-73782 · 497 bp

Back to Portulaca

Pi 0.0888

MarkerSeek score 79.90

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 497
variable_sites: 57
pi: 0.0888
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	ACCTATCCCGGATATTGCCA	ACACCCATATTCCAGGACCA	50.0	50.0	58.0	58.0	1239 bp	0.333333	52.9
clpP-psbB_p2	ACCTATCCCGGATATTGCCA	AATGCGCCAAAACCAAAACA	50.0	40.0	58.0	58.0	1201 bp	0.333333	52.8
clpP-psbB_p3	ACCTATCCCGGATATTGCCA	ATGCGCCAAAACCAAAACAA	50.0	40.0	58.0	58.0	1200 bp	0.333333	52.8
clpP-psbB_p4	TGTTCCTCCCGCTAGTACAA	ACACCCATATTCCAGGACCA	50.0	50.0	58.1	58.0	1335 bp	0.333333	52.6
clpP-psbB_p5	TCGGGTAGCACAGATCTCAT	ACACCCATATTCCAGGACCA	50.0	50.0	57.9	58.0	1271 bp	0.333333	52.6