Hotspot

rps4-trnT-UGU

LSC · 47019-47494 · 476 bp

Back to Portulaca

Pi 0.0527

MarkerSeek score 78.60

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 476
variable_sites: 32
pi: 0.0527
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	AGTTAATCCGGGCAAAGCTC	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.2	57.9	534-605 bp	1.0	85.5
rps4-trnT-UGU_p2	AGTTAATCCGGGCAAAGCTC	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.2	57.8	502-573 bp	1.0	84.9
rps4-trnT-UGU_p3	TAATCCGGGCAAAGCTCCTA	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.5	57.9	531-602 bp	1.0	83.5
rps4-trnT-UGU_p4	TAATCCGGGCAAAGCTCCTA	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.5	57.8	499-570 bp	1.0	83.0
rps4-trnT-UGU_p5	AGTTAATCCGGGCAAAGCTC	CCGCTTAGCTCAGAGGTTAG	50.0	55.0	58.2	57.5	542-613 bp	1.0	82.5