Hotspot

ycf3-trnS-GGA

LSC · 44537-45966 · 1430 bp

Back to Portulaca

Pi 0.209

MarkerSeek score 74.20

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 1430
variable_sites: 237
pi: 0.209
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.58
markerseek_score: 74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	ACTTCACCTTCAACAAGCGT	TGAACCACTCGGACATCTCT	45.0	50.0	58.0	58.1	1055-1751 bp	1.0	91.1
ycf3-trnS-GGA_p2	ACTTCACCTTCAACAAGCGT	CCCTCGGTAAACAAAAGCCT	45.0	50.0	58.0	58.1	1100-1796 bp	1.0	90.9
ycf3-trnS-GGA_p3	ACTTCACCTTCAACAAGCGT	TCCCCCGTCTCGAATTTTTC	45.0	50.0	58.0	57.9	1347-2051 bp	1.0	90.9
ycf3-trnS-GGA_p4	ACTTCACCTTCAACAAGCGT	AGTCAGGGGTTTGATCTGGA	45.0	50.0	58.0	58.0	1495-2096 bp	0.666667	78.0
ycf3-trnS-GGA_p5	ACTTCACCTTCAACAAGCGT	TTGATGCCCGTACTTTCCAG	45.0	50.0	58.0	57.9	1991 bp	0.333333	52.2