primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	GGCCTGTAGTGGGTATTTGG	20	20	55.0	55.0	58.1	57.9	0.0	0.0	0.181702	71	336	512-517	1.000000	86.3
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	GATCCACTTGGCTACATCCG	ATATTCAGCTCCGGTTGCAG	20	20	55.0	50.0	58.1	58.0	0.0	20.4	0.169878	71	336	907-912	1.000000	83.0
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	CAATCCACTGCCTTGATCCA	ATATTCAGCTCCGGTTGCAG	20	20	50.0	50.0	57.9	58.0	0.0	20.4	0.174240	71	336	921-926	1.000000	82.9
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	AATCCACTGCCTTGATCCAC	ATATTCAGCTCCGGTTGCAG	20	20	50.0	50.0	57.9	58.0	0.0	20.4	0.175747	71	336	920-925	1.000000	82.9
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	ACAATCCACTGCCTTGATCC	ATATTCAGCTCCGGTTGCAG	20	20	50.0	50.0	57.9	58.0	0.0	20.4	0.175747	71	336	922-927	1.000000	82.9
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	5882	6379	571-639	1.000000	85.5
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	CCACAACAGATCGTGTCCTT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.8	58.1	0.0	16.9	0.245933	5882	6379	592-660	1.000000	85.0
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	TAAGGCATCGGGTTTTGGTC	20	20	45.0	50.0	58.1	58.2	8.1	0.0	0.310345	5882	6379	601-669	1.000000	84.4
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	CCACAACAGATCGTGTCCTT	TAAGGCATCGGGTTTTGGTC	20	20	50.0	50.0	57.8	58.2	0.0	0.0	0.340853	5882	6379	622-690	1.000000	83.9
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	ATCCACAACAGATCGTGTCC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.6	58.1	0.0	16.9	0.462762	5882	6379	594-662	1.000000	83.2
psbI-trnS-GCU_p1	psbI-trnS-GCU	1	GTAATCCTGGACGCGAAGAA	GATGGCTGAGTGGACGAAAG	20	20	50.0	55.0	57.7	58.6	15.9	0.0	0.925217	7515	7699	291-313	1.000000	83.9
psbI-trnS-GCU_p2	psbI-trnS-GCU	2	GTAATCCTGGACGCGAAGAA	TTCGAATCCCTCTCTTTCCG	20	20	50.0	50.0	57.7	57.0	15.9	0.0	1.250487	7515	7699	228-250	1.000000	82.0
psbI-trnS-GCU_p3	psbI-trnS-GCU	3	GTAATCCTGGACGCGAAGAA	CGAAAGCGTCGGATTGCTAA	20	20	50.0	50.0	57.7	59.0	15.9	7.9	1.292499	7515	7699	277-299	1.000000	81.4
psbI-trnS-GCU_p4	psbI-trnS-GCU	4	GTAATCCTGGACGCGAAGAA	GGGTTCGAATCCCTCTCTTT	20	20	50.0	50.0	57.7	56.9	15.9	17.2	1.371516	7515	7699	231-253	1.000000	81.0
psbI-trnS-GCU_p5	psbI-trnS-GCU	5	GTAATCCTGGACGCGAAGAA	AGATGGCTGAGTGGACGAAA	20	20	50.0	50.0	57.7	59.0	15.9	0.0	1.312320	7515	7699	292-314	1.000000	80.8
trnR-UCU-atpA_p1	trnR-UCU-atpA	1	AGCCTTCCAAGCTAACGATG	GAGGAAGCAGAAGCCCTTTT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.113333	9595	9746	480-483	1.000000	83.3
trnR-UCU-atpA_p2	trnR-UCU-atpA	2	AGCCTTCCAAGCTAACGATG	GGGATCTGCGGCTCAAATTA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.062520	9595	9746	829-832	1.000000	83.0
trnR-UCU-atpA_p3	trnR-UCU-atpA	3	AGCCTTCCAAGCTAACGATG	TAAACAACCCCAATCTGCCC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.098203	9595	9746	667-670	1.000000	83.0
trnR-UCU-atpA_p4	trnR-UCU-atpA	4	CCTAGCCTTCCAAGCTAACG	TAAACAACCCCAATCTGCCC	20	20	55.0	50.0	58.1	58.1	0.0	0.0	0.127040	9595	9746	670-673	1.000000	82.8
trnR-UCU-atpA_p5	trnR-UCU-atpA	5	CCTAGCCTTCCAAGCTAACG	GGGATCTGCGGCTCAAATTA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.091356	9595	9746	832-835	1.000000	82.7
ycf3-trnS-GGA_p1	ycf3-trnS-GGA	1	ACTTCACCTTCAACAAGCGT	TGAACCACTCGGACATCTCT	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.113656	44537	45966	1055-1751	1.000000	91.1
ycf3-trnS-GGA_p2	ycf3-trnS-GGA	2	ACTTCACCTTCAACAAGCGT	CCCTCGGTAAACAAAAGCCT	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.132749	44537	45966	1100-1796	1.000000	90.9
ycf3-trnS-GGA_p3	ycf3-trnS-GGA	3	ACTTCACCTTCAACAAGCGT	TCCCCCGTCTCGAATTTTTC	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.141924	44537	45966	1347-2051	1.000000	90.9
ycf3-trnS-GGA_p4	ycf3-trnS-GGA	4	ACTTCACCTTCAACAAGCGT	AGTCAGGGGTTTGATCTGGA	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.083179	44537	45966	1495-2096	0.666667	78.0
ycf3-trnS-GGA_p5	ycf3-trnS-GGA	5	ACTTCACCTTCAACAAGCGT	TTGATGCCCGTACTTTCCAG	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.146337	44537	45966	1991-1991	0.333333	52.2
rps4-trnT-UGU_p1	rps4-trnT-UGU	1	AGTTAATCCGGGCAAAGCTC	CTCAGAGGTTAGAGCATCGC	20	20	50.0	55.0	58.2	57.9	0.0	0.0	0.381303	47019	47494	534-605	1.000000	85.5
rps4-trnT-UGU_p2	rps4-trnT-UGU	2	AGTTAATCCGGGCAAAGCTC	ATGGTCATCGGTTCGATTCC	20	20	50.0	50.0	58.2	57.8	0.0	0.0	0.489056	47019	47494	502-573	1.000000	84.9
rps4-trnT-UGU_p3	rps4-trnT-UGU	3	TAATCCGGGCAAAGCTCCTA	CTCAGAGGTTAGAGCATCGC	20	20	50.0	55.0	58.5	57.9	0.0	0.0	0.630050	47019	47494	531-602	1.000000	83.5
rps4-trnT-UGU_p4	rps4-trnT-UGU	4	TAATCCGGGCAAAGCTCCTA	ATGGTCATCGGTTCGATTCC	20	20	50.0	50.0	58.5	57.8	0.0	0.0	0.737803	47019	47494	499-570	1.000000	83.0
rps4-trnT-UGU_p5	rps4-trnT-UGU	5	AGTTAATCCGGGCAAAGCTC	CCGCTTAGCTCAGAGGTTAG	20	20	50.0	55.0	58.2	57.5	0.0	7.6	0.749072	47019	47494	542-613	1.000000	82.5
clpP_p1	clpP	1	TAACGTTCGCTACCAATGGG	AGTAGCAATACGCAATGGGG	20	20	50.0	50.0	58.0	58.0	2.8	1.6	0.045618	72686	73285	1318-2999	1.000000	88.7
clpP_p2	clpP	2	TAACGTTCGCTACCAATGGG	GTAGCAATACGCAATGGGGA	20	20	50.0	50.0	58.0	58.0	2.8	1.6	0.051143	72686	73285	1317-2998	1.000000	88.6
clpP_p3	clpP	3	TAACGTTCGCTACCAATGGG	ATGCAATGGACCCCTTTTGT	20	20	50.0	45.0	58.0	58.0	2.8	0.0	0.041498	72686	73285	1439-2912	0.666667	74.7
clpP_p4	clpP	4	TAACGTTCGCTACCAATGGG	ATACCAAGGCAAACCCATGG	20	20	50.0	50.0	58.0	58.1	2.8	4.1	0.148268	72686	73285	1524-2997	0.666667	73.5
clpP_p5	clpP	5	TAACGTTCGCTACCAATGGG	ACGCAATGGGGAGTTGATAC	20	20	50.0	50.0	58.0	58.0	2.8	1.6	0.051324	72686	73285	1309-2990	0.666667	72.8
clpP-psbB_p1	clpP-psbB	1	ACCTATCCCGGATATTGCCA	ACACCCATATTCCAGGACCA	20	20	50.0	50.0	58.0	58.0	0.7	0.0	0.059054	73286	73782	1239-1239	0.333333	52.9
clpP-psbB_p2	clpP-psbB	2	ACCTATCCCGGATATTGCCA	AATGCGCCAAAACCAAAACA	20	20	50.0	40.0	58.0	58.0	0.7	4.2	0.068701	73286	73782	1201-1201	0.333333	52.8
clpP-psbB_p3	clpP-psbB	3	ACCTATCCCGGATATTGCCA	ATGCGCCAAAACCAAAACAA	20	20	50.0	40.0	58.0	58.0	0.7	4.2	0.068701	73286	73782	1200-1200	0.333333	52.8
clpP-psbB_p4	clpP-psbB	4	TGTTCCTCCCGCTAGTACAA	ACACCCATATTCCAGGACCA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.092703	73286	73782	1335-1335	0.333333	52.6
clpP-psbB_p5	clpP-psbB	5	TCGGGTAGCACAGATCTCAT	ACACCCATATTCCAGGACCA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.093443	73286	73782	1271-1271	0.333333	52.6
rpl22_p1	rpl22	1	CGAACCGTATAGGCGCAATA	TGGGTAGAGCCGGATCTAAG	20	20	50.0	55.0	57.9	58.0	10.8	29.9	0.057889	85040	85744	1960-1972	1.000000	86.3
rpl22_p2	rpl22	2	AATTTTCGCGCGAATGGTTT	TGGGTAGAGCCGGATCTAAG	20	20	40.0	55.0	57.9	58.0	41.6	29.9	0.062115	85040	85744	1937-1949	1.000000	86.3
rpl22_p3	rpl22	3	CGAACCGTATAGGCGCAATA	GGGCCCCAATTGGTAGAAAA	20	20	50.0	50.0	57.9	58.1	10.8	22.9	0.109107	85040	85744	1861-1873	1.000000	86.2
rpl22_p4	rpl22	4	CGAACCGTATAGGCGCAATA	GAGGGTAAATCGGCCACATT	20	20	50.0	50.0	57.9	57.9	10.8	2.2	0.107470	85040	85744	2072-2084	1.000000	85.6
rpl22_p5	rpl22	5	AATTTTCGCGCGAATGGTTT	GAGGGTAAATCGGCCACATT	20	20	40.0	50.0	57.9	57.9	41.6	2.2	0.111696	85040	85744	2049-2061	1.000000	85.6
rpl2-trnI-CAU_p1	rpl2-trnI-CAU	1	AATGTGGCCGATTTACCCTC	GAATGGTTAAAGCGCCCAAC	20	20	50.0	50.0	57.9	58.0	0.0	2.3	0.061983	86985	87628	1042-1239	1.000000	91.0
rpl2-trnI-CAU_p2	rpl2-trnI-CAU	2	CTTAGATCCGGCTCTACCCA	GAATGGTTAAAGCGCCCAAC	20	20	55.0	50.0	58.0	58.0	14.5	2.3	0.012402	86985	87628	1154-1351	1.000000	90.8
rpl2-trnI-CAU_p3	rpl2-trnI-CAU	3	CTTAGATCCGGCTCTACCCA	ATTCACCATGCACGAGGATC	20	20	55.0	50.0	58.0	58.0	14.5	8.5	0.044160	86985	87628	1194-1391	1.000000	90.4
rpl2-trnI-CAU_p4	rpl2-trnI-CAU	4	CTTAGATCCGGCTCTACCCA	GGAGCGAAACAATCAGCCTA	20	20	55.0	50.0	58.0	58.0	14.5	0.0	0.027377	86985	87628	1677-1926	1.000000	90.3
rpl2-trnI-CAU_p5	rpl2-trnI-CAU	5	TTTTCTACCAATTGGGGCCC	GAATGGTTAAAGCGCCCAAC	20	20	50.0	50.0	58.1	58.0	27.9	2.3	0.063621	86985	87628	1253-1450	1.000000	89.9
trnN-GUU-ycf1_p1	trnN-GUU-ycf1	1	GGCAGAATAGCAGAGCAAGT	CCTGTGGAGAATGTGGTCAG	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.140536	108628	108973	873-1353	1.000000	86.2
trnN-GUU-ycf1_p2	trnN-GUU-ycf1	2	GAACTTGGGTGTGGGTCTTT	CCTGTGGAGAATGTGGTCAG	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.172372	108628	108973	1025-1515	1.000000	85.9
trnN-GUU-ycf1_p3	trnN-GUU-ycf1	3	GGCAGAATAGCAGAGCAAGT	AGGAAGAGATAAGAGGGCCC	20	20	50.0	55.0	58.0	57.9	0.0	16.5	0.146283	108628	108973	891-926	0.666667	70.4
trnN-GUU-ycf1_p4	trnN-GUU-ycf1	4	GGCAGAATAGCAGAGCAAGT	GGAAGAGATAAGAGGGCCCT	20	20	50.0	55.0	58.0	57.9	0.0	29.2	0.146283	108628	108973	890-925	0.666667	70.4
trnN-GUU-ycf1_p5	trnN-GUU-ycf1	5	GGCAGAATAGCAGAGCAAGT	TTCCATAACCAGAGCTCGGA	20	20	50.0	50.0	58.0	58.1	0.0	25.0	0.174889	108628	108973	913-948	0.666667	70.2
ycf1_p1	ycf1	1	TTTCCTTTTGGTTCTCCCCG	CCAGATTCGATATGGGACGAC	20	21	50.0	52.4	58.0	58.1	0.0	0.0	1.062441	112367	113366	1918-1918	0.333333	44.8
ycf1_p2	ycf1	2	GGGGAATGGAAACCGACTTT	CCAGATTCGATATGGGACGAC	20	21	50.0	52.4	58.1	58.1	0.0	0.0	1.132907	112367	113366	1935-1935	0.333333	44.3
ycf1_p3	ycf1	3	TTTCCTTTTGGTTCTCCCCG	TCCAGATTCGATATGGGACGA	20	21	50.0	47.6	58.0	58.1	0.0	10.2	1.138841	112367	113366	1919-1919	0.333333	44.2
ycf1_p4	ycf1	4	GGATACGACCTTCTCATGGC	CCAGATTCGATATGGGACGAC	20	21	55.0	52.4	57.8	58.1	0.0	0.0	1.208491	112367	113366	1998-1998	0.333333	43.7
ycf1_p5	ycf1	5	GGGGAATGGAAACCGACTTT	TCCAGATTCGATATGGGACGA	20	21	50.0	47.6	58.1	58.1	0.0	10.2	1.209307	112367	113366	1936-1936	0.333333	43.7
ndhF-rpl32_p1	ndhF-rpl32	1	GCCCATATACGCCGAAGATT	TTAACGCTGCCCAATATCCC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.119103	117259	118513	1449-1465	0.666667	72.7
ndhF-rpl32_p2	ndhF-rpl32	2	GCCAAAATCCCCTACACGAT	TTAACGCTGCCCAATATCCC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.077123	117259	118513	1891-1907	0.666667	71.7
ndhF-rpl32_p3	ndhF-rpl32	3	ACAAACGCTTTTTGACAGGC	TTAACGCTGCCCAATATCCC	20	20	45.0	50.0	58.1	58.0	13.0	0.0	0.103097	117259	118513	1866-1882	0.666667	71.5
ndhF-rpl32_p4	ndhF-rpl32	4	CAAACGCTTTTTGACAGGCA	TTAACGCTGCCCAATATCCC	20	20	45.0	50.0	58.1	58.0	2.3	0.0	0.104422	117259	118513	1865-1881	0.666667	71.5
ndhF-rpl32_p5	ndhF-rpl32	5	ACATAGGAACCGGAAGTGGA	TTAACGCTGCCCAATATCCC	20	20	50.0	50.0	58.1	58.0	9.5	0.0	0.077356	117259	118513	1404-1404	0.333333	52.7
rpl32-ccsA_p1	rpl32-ccsA	1	GGGATATTGGGCAGCGTTAA	ATCCGCATAAAAGAGCTGCA	20	20	50.0	45.0	58.0	58.0	0.0	1.0	0.067026	118688	119020	899-1640	0.666667	69.7
rpl32-ccsA_p2	rpl32-ccsA	2	GGGATATTGGGCAGCGTTAA	TCCGCATAAAAGAGCTGCAT	20	20	50.0	45.0	58.0	58.0	0.0	1.0	0.067026	118688	119020	898-1639	0.666667	69.7
rpl32-ccsA_p3	rpl32-ccsA	3	GGGCAGCGTTAAAAGCTTTT	TCCGCATAAAAGAGCTGCAT	20	20	45.0	45.0	57.9	58.0	26.5	1.0	0.191602	118688	119020	890-1631	0.666667	68.8
rpl32-ccsA_p4	rpl32-ccsA	4	GGGATATTGGGCAGCGTTAA	CGAAAGGGGAGAAGCACTTT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.126780	118688	119020	1060-1060	0.333333	52.3
rpl32-ccsA_p5	rpl32-ccsA	5	GGGATATTGGGCAGCGTTAA	GAAACGAAAGGGGAGAAGCA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.132131	118688	119020	1064-1064	0.333333	52.3
ycf1-trnN-GUU_p1	ycf1-trnN-GUU	1	CCTGTGGAGAATGTGGTCAG	GGCAGAATAGCAGAGCAAGT	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.140536	134237	134582	873-1353	1.000000	86.1
ycf1-trnN-GUU_p2	ycf1-trnN-GUU	2	CCTGTGGAGAATGTGGTCAG	GAACTTGGGTGTGGGTCTTT	20	20	55.0	50.0	57.9	57.9	0.0	0.0	0.172372	134237	134582	1025-1515	1.000000	85.9
ycf1-trnN-GUU_p3	ycf1-trnN-GUU	3	AGGAAGAGATAAGAGGGCCC	GGCAGAATAGCAGAGCAAGT	20	20	55.0	50.0	57.9	58.0	16.5	0.0	0.146283	134237	134582	891-926	0.666667	70.6
ycf1-trnN-GUU_p4	ycf1-trnN-GUU	4	GGAAGAGATAAGAGGGCCCT	GGCAGAATAGCAGAGCAAGT	20	20	55.0	50.0	57.9	58.0	29.2	0.0	0.146283	134237	134582	890-925	0.666667	70.6
ycf1-trnN-GUU_p5	ycf1-trnN-GUU	5	TTCCATAACCAGAGCTCGGA	GGCAGAATAGCAGAGCAAGT	20	20	50.0	50.0	58.1	58.0	25.0	0.0	0.174889	134237	134582	913-948	0.666667	70.3
trnI-CAU-trnH-GUG_p1	trnI-CAU-trnH-GUG	1	CAACGGGACCCTCCAATAAG	AGAAGAGTTCGACCCAATGC	20	20	55.0	50.0	58.0	57.9	9.4	0.0	0.138992	155794	155913	289-471	1.000000	90.9
trnI-CAU-trnH-GUG_p2	trnI-CAU-trnH-GUG	2	CAACGGGACCCTCCAATAAG	GAAGAGTTCGACCCAATGCT	20	20	55.0	50.0	58.0	57.9	9.4	0.0	0.138992	155794	155913	288-470	1.000000	90.9
trnI-CAU-trnH-GUG_p3	trnI-CAU-trnH-GUG	3	AACGGGACCCTCCAATAAGT	AGAAGAGTTCGACCCAATGC	20	20	50.0	50.0	58.3	57.9	9.4	0.0	0.435467	155794	155913	288-470	1.000000	88.5
trnI-CAU-trnH-GUG_p4	trnI-CAU-trnH-GUG	4	AACGGGACCCTCCAATAAGT	GAAGAGTTCGACCCAATGCT	20	20	50.0	50.0	58.3	57.9	9.4	0.0	0.435467	155794	155913	287-469	1.000000	88.5
trnI-CAU-trnH-GUG_p5	trnI-CAU-trnH-GUG	5	CAACGGGACCCTCCAATAAG	CCAAAGAAGAGTTCGACCCA	20	20	55.0	50.0	58.0	57.5	9.4	0.0	0.582164	155794	155913	293-475	1.000000	87.3