Hotspot

ycf23

Genome · 163550-164347 · 798 bp

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Pi 0.118

MarkerSeek score 79.30

Primer available yes

Label ycf23

Feature Metadata

label_name: ycf23
feature_type: gene
region: Genome
length_bp: 798
variable_sites: 94
pi: 0.118
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf23_p1	TGTGCCTTCTTGAATTCCGT	TTTGGCAAGTAGTTCCACCC	45.0	50.0	57.7	58.0	1619-1625 bp	1.0	86.3
ycf23_p2	TGTGCCTTCTTGAATTCCGT	GCAAGTAGTTCCACCCTCTG	45.0	55.0	57.7	57.9	1615-1621 bp	1.0	85.6
ycf23_p3	TGTGCCTTCTTGAATTCCGT	AGAAATGGTGCCTTGGGATG	45.0	50.0	57.7	58.1	1315-1318 bp	1.0	85.0
ycf23_p4	TGTGCCTTCTTGAATTCCGT	TTCTGCACCGAGAACAGAAG	45.0	50.0	57.7	57.8	1273-1276 bp	1.0	85.0
ycf23_p5	ATAGTTAAGGCCCCTTTCGC	TTTGGCAAGTAGTTCCACCC	50.0	50.0	57.7	58.0	1919 bp	0.5	57.2