Hotspot

ycf22

Genome · 162844-163473 · 630 bp

Back to Porphyra

Pi 0.114

MarkerSeek score 79.00

Primer available yes

Label ycf22

Feature Metadata

label_name: ycf22
feature_type: gene
region: Genome
length_bp: 630
variable_sites: 72
pi: 0.114
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf22_p1	AGGATCATCTGCTCCTCCTC	TCGCGTTTCTTTATGGAGCA	55.0	45.0	58.0	57.9	1227 bp	0.5	59.2
ycf22_p2	GGATCATCTGCTCCTCCTCT	TCGCGTTTCTTTATGGAGCA	55.0	45.0	58.0	57.9	1226 bp	0.5	59.2
ycf22_p3	AGGAACTGTTGGTTTGCGAT	TCGCGTTTCTTTATGGAGCA	45.0	45.0	58.0	57.9	957 bp	0.5	59.2
ycf22_p4	AGGATCATCTGCTCCTCCTC	TACCACATGCTACACTGTGC	55.0	50.0	58.0	57.9	1485 bp	0.5	59.1
ycf22_p5	GGATCATCTGCTCCTCCTCT	TACCACATGCTACACTGTGC	55.0	50.0	58.0	57.9	1484 bp	0.5	59.1