Hotspot

ycf16

Genome · 42408-43163 · 756 bp

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Pi 0.107

MarkerSeek score 79.70

Primer available yes

Label ycf16

Feature Metadata

label_name: ycf16
feature_type: gene
region: Genome
length_bp: 756
variable_sites: 81
pi: 0.107
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf16_p1	AGGTTGTACAGCTCCTCAGT	TCGGATCAGCTGCTCAGATA	50.0	50.0	58.0	58.0	1979 bp	1.0	86.6
ycf16_p2	AGGTTGTACAGCTCCTCAGT	CGTTTTCGCAGTTTGCATCT	50.0	45.0	58.0	58.0	1895 bp	1.0	86.5
ycf16_p3	AGGTTGTACAGCTCCTCAGT	CCCGTTGAAGAACAAACAGC	50.0	50.0	58.0	57.9	1791 bp	1.0	86.0
ycf16_p4	GAAATAAGGACGGCAAGGGT	TCGGATCAGCTGCTCAGATA	50.0	50.0	57.9	58.0	1866 bp	0.5	58.9
ycf16_p5	AGGTTGTACAGCTCCTCAGT	CAGGACGTGAAGCATATCCC	50.0	55.0	58.0	58.1	2234 bp	0.5	58.8