Hotspot

rps1

Genome · 132858-133649 · 792 bp

Back to Porphyra

Pi 0.0997

MarkerSeek score 79.50

Primer available yes

Label rps1

Feature Metadata

label_name: rps1
feature_type: gene
region: Genome
length_bp: 792
variable_sites: 79
pi: 0.0997
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps1_p1	ATATAGGGCGAATGACGGGA	GCGGTTTATTTCTGTGGCAC	50.0	50.0	58.1	58.0	1457 bp	1.0	86.6
rps1_p2	ATATAGGGCGAATGACGGGA	ACATAAGCCGGGTTCTGTTC	50.0	50.0	58.1	57.9	1699-1700 bp	1.0	85.5
rps1_p3	ATATAGGGCGAATGACGGGA	TGCTCCGTTTTTGGGGTTTA	50.0	45.0	58.1	57.9	1556 bp	0.5	58.9
rps1_p4	ATATAGGGCGAATGACGGGA	ATTTAGTCGCTTTGCTCCGT	50.0	45.0	58.1	57.9	1568 bp	0.5	58.7
rps1_p5	ATATAGGGCGAATGACGGGA	GTGGCACGTTCCTCATAGTT	50.0	50.0	58.1	57.9	1444 bp	0.5	58.7