Hotspot

ftsH

Genome · 114350-116236 · 1887 bp

Back to Porphyra

Pi 0.0514

MarkerSeek score 72.10

Primer available yes

Label ftsH

Feature Metadata

label_name: ftsH
feature_type: gene
region: Genome
length_bp: 1887
variable_sites: 97
pi: 0.0514
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ftsH_p1	TTGTTGCAATCAGCCACTCT	AGTGGTTAGAGCAGGGGATT	45.0	50.0	58.0	58.0	2623-2637 bp	1.0	85.3
ftsH_p2	TTGTTGCAATCAGCCACTCT	TGGTTAGAGCAGGGGATTCA	45.0	50.0	58.0	58.0	2621-2635 bp	1.0	85.3
ftsH_p3	GTCCATTGCTGCAACAGTTC	AGTGGTTAGAGCAGGGGATT	50.0	50.0	57.9	58.0	2866-2880 bp	1.0	85.1
ftsH_p4	GTCCATTGCTGCAACAGTTC	TGGTTAGAGCAGGGGATTCA	50.0	50.0	57.9	58.0	2864-2878 bp	1.0	85.0
ftsH_p5	TTGTTGCAATCAGCCACTCT	GAGCAGGGGATTCATAAGCC	45.0	55.0	58.0	58.1	2615-2629 bp	1.0	84.8