Hotspot

dnaB

Genome · 123618-125324 · 1707 bp

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Pi 0.135

MarkerSeek score 79.00

Primer available yes

Label dnaB

Feature Metadata

label_name: dnaB
feature_type: gene
region: Genome
length_bp: 1707
variable_sites: 230
pi: 0.135
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
dnaB_p1	TCAGTTGGTAGAGCAGTGGA	CGGCCACCTCTCCTATAAGA	50.0	55.0	58.0	58.0	2518 bp	0.5	59.9
dnaB_p2	TCAGTTGGTAGAGCAGTGGA	CTCGGCCACCTCTCCTATAA	50.0	55.0	58.0	58.0	2520 bp	0.5	59.9
dnaB_p3	TCAGTTGGTAGAGCAGTGGA	TCGGCCACCTCTCCTATAAG	50.0	55.0	58.0	58.0	2519 bp	0.5	59.9
dnaB_p4	CCTCGTGTCACCAGTTCAAA	CGGCCACCTCTCCTATAAGA	50.0	55.0	58.1	58.0	2490 bp	0.5	59.6
dnaB_p5	CCTCGTGTCACCAGTTCAAA	TCGGCCACCTCTCCTATAAG	50.0	55.0	58.1	58.0	2491 bp	0.5	59.6