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ORF263

Genome · 16487-17278 · 792 bp

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Pi 0.109

MarkerSeek score 79.40

Primer available yes

Label ORF263

Feature Metadata

label_name: ORF263
feature_type: gene
region: Genome
length_bp: 792
variable_sites: 86
pi: 0.109
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ORF263_p1	ACCAGTTTTAGCAGCTGCAT	CCGTTACTTGTTCACCACCA	45.0	50.0	58.1	58.0	1870 bp	0.5	59.0
ORF263_p2	ACCAGTTTTAGCAGCTGCAT	GAATCTCGGCCATTCCTTGT	45.0	50.0	58.1	58.0	1820 bp	0.5	59.0
ORF263_p3	ACCAGTTTTAGCAGCTGCAT	CGGCCATTCCTTGTACTACC	45.0	55.0	58.1	58.1	1814 bp	0.5	58.9
ORF263_p4	ACCAGTTTTAGCAGCTGCAT	AATTGATTGCCCTGGCTCAA	45.0	45.0	58.1	58.1	1513 bp	0.5	58.9
ORF263_p5	ACCAGTTTTAGCAGCTGCAT	CCTGGCTCAACGGGTATATG	45.0	55.0	58.1	57.8	1503 bp	0.5	58.0