Hotspot

psbA-ycf35

Genome · 20903-21216 · 314 bp

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Pi 0.121

MarkerSeek score 80.20

Primer available yes

Label psbA-ycf35

Feature Metadata

label_name: psbA-ycf35
feature_type: igs
region: Genome
length_bp: 314
variable_sites: 37
pi: 0.121
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-ycf35_p1	TCCAAGAGCAGAAACGTTCC	AGCCTAAATCTGTTAAAGCATGC	50.0	39.1	58.1	57.7	447-451 bp	1.0	61.2
psbA-ycf35_p2	TCCAAGAGCAGAAACGTTCC	GCCTAAATCTGTTAAAGCATGCT	50.0	39.1	58.1	57.7	446-450 bp	1.0	61.2
psbA-ycf35_p3	AAGAGCAGAAACGTTCCCAC	AGCCTAAATCTGTTAAAGCATGC	50.0	39.1	58.4	57.7	444-448 bp	1.0	59.0
psbA-ycf35_p4	AAGAGCAGAAACGTTCCCAC	GCCTAAATCTGTTAAAGCATGCT	50.0	39.1	58.4	57.7	443-447 bp	1.0	59.0
psbA-ycf35_p5	CAAGAGCAGAAACGTTCCCA	GCCTAAATCTGTTAAAGCATGCT	50.0	39.1	58.4	57.7	444-448 bp	1.0	59.0