Hotspot

thiG

Genome · 111960-112766 · 807 bp

Back to Porosira

Pi 0.117

MarkerSeek score 80.50

Primer available yes

Label thiG

Feature Metadata

label_name: thiG
feature_type: gene
region: Genome
length_bp: 807
variable_sites: 94
pi: 0.117
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
thiG_p1	AGGAGAAGGTTCAGGGTGAA	TGGTCGTAGGTTCAAGTCCT	50.0	50.0	57.9	58.0	1515 bp	0.5	59.0
thiG_p2	ACAGAAACAACGCTCGGAAT	TGGTCGTAGGTTCAAGTCCT	45.0	50.0	58.1	58.0	1064 bp	0.5	59.0
thiG_p3	TCAGTATTACCACCTGCCGA	TGGTCGTAGGTTCAAGTCCT	50.0	50.0	58.1	58.0	1579 bp	0.5	58.8
thiG_p4	AGGAGAAGGTTCAGGGTGAA	TTCAAGTCCTACCTGGGGAG	50.0	55.0	57.9	58.0	1505 bp	0.5	58.7
thiG_p5	ACAGAAACAACGCTCGGAAT	TTCAAGTCCTACCTGGGGAG	45.0	55.0	58.1	58.0	1054 bp	0.5	58.7